Repeated cycles of amplification result in degradation of your probe, causing improved fluorescence. TaqMan detection is, for that reason, more precise than dye‐centered detection, since an increase in fluorescence takes place only on binding of your probe to a selected sequence and subsequent de‐quenching, when no signal is produced by https://www.facebook.com/NewTechFind/posts/pfbid08kNHK34sDFU7xaUxbKJppUC6DVDMTxYZRjyufuQVpcFy2MaTi9iQocW6ofqpHCHdl